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pTripZ RB1 WT-3XFLAG
(Plasmid #209070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTripZ NEO DEST
  • Backbone manufacturer
    modified from thermofisher
  • Backbone size w/o insert (bp) 11676
  • Total vector size (bp) 12736
  • Modifications to backbone
    LR reaction with pENTR223 RB1 WT to make pTripz RB1 WT-3X FLAG
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
  • Alt name
    pTripZ RB1 WT 3X FLAG
  • Species
    H. sapiens (human); RB1 WT
  • Insert Size (bp)
    2876
  • GenBank ID
    5925
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTripZ RB1 WT-3XFLAG was a gift from Matthew Oser (Addgene plasmid # 209070 ; http://n2t.net/addgene:209070 ; RRID:Addgene_209070)

  • For your References section:

    Cells Lacking the RB1 Tumor Suppressor Gene Are Hyperdependent on Aurora B Kinase for Survival. Oser MG, Fonseca R, Chakraborty AA, Brough R, Spektor A, Jennings RB, Flaifel A, Novak JS, Gulati A, Buss E, Younger ST, McBrayer SK, Cowley GS, Bonal DM, Nguyen QD, Brulle-Soumare L, Taylor P, Cairo S, Ryan CJ, Pease EJ, Maratea K, Travers J, Root DE, Signoretti S, Pellman D, Ashton S, Lord CJ, Barry ST, Kaelin WG Jr. Cancer Discov. 2019 Feb;9(2):230-247. doi: 10.1158/2159-8290.CD-18-0389. Epub 2018 Oct 29. 10.1158/2159-8290.CD-18-0389 PubMed 30373918
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