Skip to main content
Addgene

pCSP1-mp53AS-6xMUT
(Plasmid #20904)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCSP1
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53AS
  • Alt name
    TP53, AS c-terminal isoform
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Mutation
    point mutation (Ser/Thr-Ala) of the 6 N-terminal phosphorylated residues (S4, S6, S9, S15, T18, S20). 5' natural EcoRV and SacI restriction sites eliminated for mutagenesis purposes.
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSP1-mp53AS-6xMUT was a gift from Stefano Piccolo (Addgene plasmid # 20904 ; http://n2t.net/addgene:20904 ; RRID:Addgene_20904)

  • For your References section:

    Integration of TGF-beta and Ras/MAPK signaling through p53 phosphorylation. Cordenonsi M, Montagner M, Adorno M, Zacchigna L, Martello G, Mamidi A, Soligo S, Dupont S, Piccolo S. Science. 2007 Feb 9. 315(5813):840-3. 10.1126/science.1135961 PubMed 17234915
Related items:
Commonly requested with: