pCS2-FLAG-hEcto/Tif1g-CAmut
(Plasmid #20903)

• Depositing Lab: Stefano Piccolo
• Publication: Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ectodermin
• Alt name: Tif1gamma
• Alt name: Transcriptional Intermediary Factor 1 gamma
• Alt name: TRIM33
• Species: H. sapiens (human)
• Insert Size (bp): 3700
• Mutation: Point mutagenesis of C125 and C128 to Alanine (RING finger mutant). Bears an additional SfiI restriction site across the mutated Cysteines. N-terminal deletion of 37 aminoacids; Ectodermin protein sequence starts with PPLTAVLE...
• Entrez Gene: TRIM33 (a.k.a. ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StuI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Subcloning of a SacI-BamHI fragment from the original pFLAG-CMV2 construct by Dr. F.J. Rauscher III

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry