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Addgene

pCS2-FLAG-hEcto/Tif1g
(Plasmid #20902)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ectodermin
  • Alt name
    Tif1gamma
  • Alt name
    Transcriptional Intermediary Factor 1 gamma
  • Alt name
    TRIM33
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • Mutation
    N-terminal deletion of 37 aminoacids; Ectodermin protein sequence starts with PPLTAVLE...
  • Entrez Gene
    TRIM33 (a.k.a. ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Subcloning of a SacI-BamHI fragment from the original pFLAG-CMV2 construct by Dr. F.J. Rauscher III
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the sequence provided covers the 5' of the construct (pCS2 polylinker, FLAG epitope-beginning of Ecto CDS)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-FLAG-hEcto/Tif1g was a gift from Stefano Piccolo (Addgene plasmid # 20902 ; http://n2t.net/addgene:20902 ; RRID:Addgene_20902)

  • For your References section:

    Germ-layer specification and control of cell growth by Ectodermin, a Smad4 ubiquitin ligase. Dupont S, Zacchigna L, Cordenonsi M, Soligo S, Adorno M, Rugge M, Piccolo S. Cell. 2005 Apr 8. 121(1):87-99. 10.1016/j.cell.2005.01.033 PubMed 15820681
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