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Addgene

C-5545 ∆cosδε
(Bacterial strain #209018)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 209018 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    C-5545 ∆cosδε
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Deficient in the P2 bacteriophage packaging cos site; rhamnose-inducible P4 delta (δ) and epsilon (ε) genes
  • Species
    Escherichia coli

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

P2 bacteriophage lysogen derived from E. coli EMG C-5545 strain.
We designed a pair of primers to check the presence of P2 lysogen in this strain:
- P2 gpH C-ter gpG Fw: 5’ GCAGAGCACGAACAGTCACG 3’
- P2 gpH C-ter gpG Rv: 5’ TTATTGCGGCATTTCCGGCC 3’
These primers amplify the C-terminal region of the P2 gpH gene (tail fiber) and the complete gpG gene (tail fiber chaperone) (PCR fragment size: 2070 bp).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C-5545 ∆cosδε was a gift from Alfonso Jaramillo (Addgene plasmid # 209018)

  • For your References section:

    Engineering Bacteria to Produce Pure Phage-like Particles for Gene Delivery. Tridgett M, Ababi M, Osgerby A, Ramirez Garcia R, Jaramillo A. ACS Synth Biol. 2021 Jan 15;10(1):107-114. doi: 10.1021/acssynbio.0c00467. Epub 2020 Dec 14. 10.1021/acssynbio.0c00467 PubMed 33317264
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