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Addgene

pETmsGFPSyt1(80 JuxtaK-142)
(Plasmid #209013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28 a (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syt1
  • Species
    R. norvegicus (rat)
  • Mutation
    aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKEK95->NQCLFQSTNNTQGSEN)
  • Entrez Gene
    Syt1 (a.k.a. P65)
  • Tag / Fusion Protein
    • 6xHis-msfGFP (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Juxta K mutant neutralizing the juxtamembrane lysine residues. Please visit https://doi.org/10.1101/2023.08.11.551903 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETmsGFPSyt1(80 JuxtaK-142) was a gift from Edwin Chapman (Addgene plasmid # 209013 ; http://n2t.net/addgene:209013 ; RRID:Addgene_209013)

  • For your References section:

    The juxtamembrane linker of synaptotagmin 1 regulates Ca(2+) binding via liquid-liquid phase separation. Mehta N, Mondal S, Watson ET, Cui Q, Chapman ER. Nat Commun. 2024 Jan 4;15(1):262. doi: 10.1038/s41467-023-44414-5. 10.1038/s41467-023-44414-5 PubMed 38177243
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