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Addgene

pCSP1-HA-xEcto
(Plasmid #20900)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSP1
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ectodermin
  • Alt name
    Tif1gamma
  • Alt name
    Transcriptional Intermediary Factor 1 gamma
  • Alt name
    TRIM33
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    4000
  • Mutation
    contains full unsequenced 3' UTR
  • Entrez Gene
    trim33 (a.k.a. ecto, ptc7, rfg7, tf1g, tif1g, tif1gamma, tifgamma)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For Xenopus embryo mRNA microinjections, cut AscI and transcribe SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSP1-HA-xEcto was a gift from Stefano Piccolo (Addgene plasmid # 20900 ; http://n2t.net/addgene:20900 ; RRID:Addgene_20900)

  • For your References section:

    Germ-layer specification and control of cell growth by Ectodermin, a Smad4 ubiquitin ligase. Dupont S, Zacchigna L, Cordenonsi M, Soligo S, Adorno M, Rugge M, Piccolo S. Cell. 2005 Apr 8. 121(1):87-99. 10.1016/j.cell.2005.01.033 PubMed 15820681
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