pDONR221-kcnk5b
(Plasmid
#208989)
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PurposeRiboprobe generation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDORN221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2551
- Total vector size (bp) 3898
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekcnk5b
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Alt nameTASK-2b
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Alt namealf, another longfin
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Alt namezgc:63921
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1347
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GenBank IDNM_200633.1
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Entrez Genekcnk5b (a.k.a. kcnk5, zgc:63921)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-kcnk5b was a gift from GuangJun Zhang (Addgene plasmid # 208989 ; http://n2t.net/addgene:208989 ; RRID:Addgene_208989) -
For your References section:
Evolution of two-pore domain potassium channels and their gene expression in zebrafish embryos. Park SJ, Silic MR, Staab PL, Chen J, Zackschewski EL, Zhang G. Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.690. 10.1002/dvdy.690 PubMed 38270285