pENTR-D-Topo-kcnk4a
(Plasmid
#208987)
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PurposeRiboprobe generation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR-D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
- Total vector size (bp) 4038
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekcnk4a
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Alt namesi:dkeyp-115e12.3
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Alt nametraak
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1458
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GenBank IDXM_021481306.1
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Entrez Genekcnk4a (a.k.a. si:dkeyp-115e12.3, traak)
- Promoter T7
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-D-Topo-kcnk4a was a gift from GuangJun Zhang (Addgene plasmid # 208987 ; http://n2t.net/addgene:208987 ; RRID:Addgene_208987) -
For your References section:
Evolution of two-pore domain potassium channels and their gene expression in zebrafish embryos. Park SJ, Silic MR, Staab PL, Chen J, Zackschewski EL, Zhang G. Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.690. 10.1002/dvdy.690 PubMed 38270285