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Addgene

pDL
(Plasmid #208978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPSup
  • Backbone manufacturer
    Jores et al, 2020
  • Backbone size (bp) 5464
  • Modifications to backbone
    The GFP reporter gene in pPSup was replaced with a nanoluciferase gene controlled by the 35S minimal promoter followed by the synJ 5′ UTR. A luciferase gene driven by the Arabidopsis UBQ10 promoter was inserted between the T-DNA left border and the BlpR gene.
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL was a gift from Stan Fields (Addgene plasmid # 208978 ; http://n2t.net/addgene:208978 ; RRID:Addgene_208978)

  • For your References section:

    Plant enhancers exhibit both cooperative and additive interactions among their functional elements. Jores T, Tonnies J, Mueth NA, Romanowski A, Fields S, Cuperus JT, Queitsch C. Plant Cell. 2024 Mar 21:koae088. doi: 10.1093/plcell/koae088. 10.1093/plcell/koae088 PubMed 38513612
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