Skip to main content
Addgene

pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
(Plasmid #208977)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pegRNA entry vector (MNW320)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
  • Alt name
    MNW320
  • gRNA/shRNA sequence
    n/a
  • Species
    Synthetic
  • Promoter human U6
  • Tag / Fusion Protein
    • BPNLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer os280-CAGGGTTATTGTCTCATGAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320) was a gift from Benjamin Kleinstiver (Addgene plasmid # 208977 ; http://n2t.net/addgene:208977 ; RRID:Addgene_208977)

  • For your References section:

    Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases. Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, Kleinstiver BP. Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x. 10.1038/s41587-024-02324-x PubMed 39039307
Related items: