pCSP1-mp53AS
(Plasmid #20896)

• Depositing Lab: Stefano Piccolo
• Publication: Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Backbone

• Vector backbone: pCSP1
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53AS
• Alt name: TP53, AS c-terminal isoform
• Species: M. musculus (mouse)
• Insert Size (bp): 1800
• Mutation: 5' natural EcoRV and SacI restriction sites eliminated for mutagenesis purposes.
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry