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Addgene

Mix.2-lux M-p53BE
(Plasmid #20894)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3basic
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    -290 Mix.2 M-p53BE promoter luciferase
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    290
  • Mutation
    point mutation of the p53 binding element (p53BE). Bears an additional EcoRI restriction site across the mutated sequence.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mix.2-lux M-p53BE was a gift from Stefano Piccolo (Addgene plasmid # 20894 ; http://n2t.net/addgene:20894 ; RRID:Addgene_20894)

  • For your References section:

    Links between tumor suppressors: p53 is required for TGF-beta gene responses by cooperating with Smads. Cordenonsi M, Dupont S, Maretto S, Insinga A, Imbriano C, Piccolo S. Cell. 2003 May 2. 113(3):301-14. 10.1016/S0092-8674(03)00308-8 PubMed 12732139
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