Skip to main content
Addgene

pCHA_OROV_NSs
(Plasmid #208939)

Print

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 208939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    V27 pLPS-3' HA - Acceptor
  • Backbone manufacturer
    Tony Pawson
  • Backbone size w/o insert (bp) 4195
  • Total vector size (bp) 4468
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NSs
  • Alt name
    Small non-structural protein
  • Species
    Oropouche orthobunyavirus
  • Insert Size (bp)
    273
  • Mutation
    Deleted 66 bp from 5' end and deleted 415 bp from 3' end so only NSs expressed
  • GenBank ID
    NC_005777.1
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    OROV genes were cloned from plasmids provided by Prof. Richard Elliott, University of Glasgow.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCHA_OROV_NSs was a gift from Gerald Barry (Addgene plasmid # 208939 ; http://n2t.net/addgene:208939 ; RRID:Addgene_208939)
Related items:
Commonly requested with: