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PDE4B-Sensor
(Plasmid #208929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet-MBP
  • Backbone manufacturer
    Attila Reményi lab
  • Backbone size w/o insert (bp) 4736
  • Total vector size (bp) 4901
  • Modifications to backbone
    PDE4B docking sequence (NheI-NdeI) + phospho site (SalI-NotI) and SmBit was cloned with NotI-XhoI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PDE4B docking sequence+Phospho site and Smbit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • GenBank ID
    NM_002600.4
  • Entrez Gene
    PDE4B (a.k.a. DPDE4, PDEIVB)
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • Smbit (C terminal on insert)
    • HISx6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer malE
  • 3′ sequencing primer T7_term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PDE4B-Sensor was a gift from Attila Reményi (Addgene plasmid # 208929 ; http://n2t.net/addgene:208929 ; RRID:Addgene_208929)

  • For your References section:

    Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding. Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A. Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. 10.3390/ijms241914854 PubMed 37834301
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