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Addgene

VF-Sensor
(Plasmid #208927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet-MBP
  • Backbone manufacturer
    Attila Reményi lab
  • Backbone size w/o insert (bp) 4730
  • Total vector size (bp) 4976
  • Modifications to backbone
    cloning of Smbit (40 bp) with NotI and XhoI, then cloning of SOS1 fragment with BamHI and NotI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SOS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    198
  • GenBank ID
    NM_005633.3
  • Entrez Gene
    SOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • Smbit (C terminal on insert)
    • HISx6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI, XhoI (not destroyed)
  • 5′ sequencing primer malE
  • 3′ sequencing primer T7_term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VF-Sensor was a gift from Attila Reményi (Addgene plasmid # 208927 ; http://n2t.net/addgene:208927 ; RRID:Addgene_208927)

  • For your References section:

    Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding. Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A. Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. 10.3390/ijms241914854 PubMed 37834301
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