CB2R-GFP
(Plasmid
#208919)
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Purposeencodes the human cannabinoid receptor 2, tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCNR2
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SpeciesH. sapiens (human)
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Insert Size (bp)1080
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Entrez GeneCNR2 (a.k.a. CB-2, CB2, CX5)
- Promoter MCV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CB2R-GFP was a gift from Christian Gruber (Addgene plasmid # 208919 ; http://n2t.net/addgene:208919 ; RRID:Addgene_208919) -
For your References section:
Discovery and development of macrocyclic peptide modulators of the cannabinoid 2 receptor. Tomašević N, Emser FS, Muratspahic E, Gattringer J, Hasinger S, Hellinger R, Keov P, Felkl M, Gertsch J, Becker CFW, Gruber CW. J Biol Chem. 2024 Jun;300(6):107330. doi: 10.1016/j.jbc.2024.107330. Epub 2024 Apr 26. 10.1016/j.jbc.2024.107330 PubMed 38679329