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Addgene

V2R-GFP
(Plasmid #208910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AVPR2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1113
  • Entrez Gene
    AVPR2 (a.k.a. ADHR, DI1, DIR, DIR3, NDI, NDI1, V2R)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V2R-GFP was a gift from Christian Gruber (Addgene plasmid # 208910 ; http://n2t.net/addgene:208910 ; RRID:Addgene_208910)

  • For your References section:

    Development of a human vasopressin V(1a)-receptor antagonist from an evolutionary-related insect neuropeptide. Di Giglio MG, Muttenthaler M, Harpsoe K, Liutkeviciute Z, Keov P, Eder T, Rattei T, Arrowsmith S, Wray S, Marek A, Elbert T, Alewood PF, Gloriam DE, Gruber CW. Sci Rep. 2017 Feb 1;7:41002. doi: 10.1038/srep41002. 10.1038/srep41002 PubMed 28145450
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