Vd-ARATR-GFP
(Plasmid
#208903)
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Purposeencodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARATR
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SpeciesVarroa destructor
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Insert Size (bp)1059
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Vd-ARATR-GFP was a gift from Christian Gruber (Addgene plasmid # 208903 ; http://n2t.net/addgene:208903 ; RRID:Addgene_208903) -
For your References section:
I8-arachnotocin-an arthropod-derived G protein-biased ligand of the human vasopressin V(2) receptor. Duerrauer L, Muratspahic E, Gattringer J, Keov P, Mendel HC, Pfleger KDG, Muttenthaler M, Gruber CW. Sci Rep. 2019 Dec 17;9(1):19295. doi: 10.1038/s41598-019-55675-w. 10.1038/s41598-019-55675-w PubMed 31848378