Tc-INTR-GFP
(Plasmid
#208899)
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Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the beetle Tribolium castaneum, tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINTR
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SpeciesTribolium castaneum
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Insert Size (bp)1187
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site AgeI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tc-INTR-GFP was a gift from Christian Gruber (Addgene plasmid # 208899 ; http://n2t.net/addgene:208899 ; RRID:Addgene_208899) -
For your References section:
Discovery of peptide probes to modulate oxytocin-type receptors of insects. Keov P, Liutkeviciute Z, Hellinger R, Clark RJ, Gruber CW. Sci Rep. 2018 Jul 3;8(1):10020. doi: 10.1038/s41598-018-28380-3. 10.1038/s41598-018-28380-3 PubMed 29968789