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Addgene

BAT-gal
(Plasmid #20889)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7XTCF binding sites, Siamois minimal promoter, NLS-lacz
  • Species
    X. laevis (frog); E. coli
  • Insert Size (bp)
    3700
  • Mutation
    7 multimerized TCF-binding elements cloned upstream of the xSiamois minimal promoter, driving expression of NLS-lacZ (nuclear localized beta-galactosidase)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Wnt/beta-catenin responsive promoter in mouse tissues (used to derive the BAT-gal mouse strain), Xenopus embryos and human cell lines

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAT-gal was a gift from Stefano Piccolo (Addgene plasmid # 20889 ; http://n2t.net/addgene:20889 ; RRID:Addgene_20889)

  • For your References section:

    Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors. Maretto S, Cordenonsi M, Dupont S, Braghetta P, Broccoli V, Hassan AB, Volpin D, Bressan GM, Piccolo S. Proc Natl Acad Sci U S A. 2003 Mar 18. 100(6):3299-304. 10.1073/pnas.0434590100 PubMed 12626757
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