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Addgene

pAbaMCi-GFP-BsaI_pJMP2748
(Plasmid #208888)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208888 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    R6K
  • Vector type
    Bacterial Expression ; pir dependent origin of replication

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Growth instructions
    pir dependent origin of replication
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9, sgRNA expression cassette, sfGFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAbaMCi-GFP-BsaI_pJMP2748 was a gift from Jason Peters (Addgene plasmid # 208888 ; http://n2t.net/addgene:208888 ; RRID:Addgene_208888)
  • For your References section:

    Essential gene knockdowns reveal genetic vulnerabilities and antibiotic sensitivities in Acinetobacter baumannii. Ward RD, Tran JS, Banta AB, Bacon EE, Rose WE, Peters JM. mBio. 2023 Dec 21:e0205123. doi: 10.1128/mbio.02051-23. 10.1128/mbio.02051-23 PubMed 38126769