mito-PDCD10-mScarlet-I
(Plasmid
#208887)
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PurposeExpress mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208887 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTS_mScarlet-i_N1
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Backbone manufacturerAddgene plasmid # 85059 ; http://n2t.net/addgene:85059 ; RRID:Addgene_85059
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCCM3
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SpeciesH. sapiens (human)
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MutationPDCD10 targeted to the mitonchondria
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Entrez GenePDCD10 (a.k.a. CCM3, TFAR15)
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Tag
/ Fusion Protein
- mScarlet-I
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe mito-PDCD10-mScarlet-I construct was created by inserting a custom gene block (IDT) in the pMTS_mScarlet-i_N1 plasmid (Addgene #85059) using the XhoI/EcoRI sites.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mito-PDCD10-mScarlet-I was a gift from Guillaume Jacquemet (Addgene plasmid # 208887 ; http://n2t.net/addgene:208887 ; RRID:Addgene_208887) -
For your References section:
TLNRD1 is a CCM complex component and regulates endothelial barrier integrity. Neil J. Ball, Sujan Ghimire, Gautier Follain, Ada O. Pajari, Monika Vaitkevičiūtė, Alana R. Cowell, Bence Berki, Johanna Ivaska, ProfileIlkka Paatero, Benjamin T. Goult, Guillaume Jacquemet. BioRxiv 10.1101/2023.09.29.559344