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Addgene

mito-CCM2-mScarlet-I
(Plasmid #208886)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTS_mScarlet-i_N1
  • Backbone manufacturer
    Addgene plasmid # 85059 ; http://n2t.net/addgene:85059 ; RRID:Addgene_85059
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCM2
  • Species
    H. sapiens (human)
  • Mutation
    CCM2 targeted to the mitonchondria
  • Entrez Gene
    CCM2 (a.k.a. C7orf22, OSM, PP10187)
  • Tag / Fusion Protein
    • mScarlet-I

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mito-CCM2-mScarlet-I construct was created by inserting a custom gene block (IDT) in the pMTS_mScarlet-i_N1 plasmid (Addgene #85059) using the XhoI/EcoRI sites.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mito-CCM2-mScarlet-I was a gift from Guillaume Jacquemet (Addgene plasmid # 208886 ; http://n2t.net/addgene:208886 ; RRID:Addgene_208886)

  • For your References section:

    TLNRD1 is a CCM complex component and regulates endothelial barrier integrity. Neil J. Ball, Sujan Ghimire, Gautier Follain, Ada O. Pajari, Monika Vaitkevičiūtė, Alana R. Cowell, Bence Berki, Johanna Ivaska, ProfileIlkka Paatero, Benjamin T. Goult, Guillaume Jacquemet. BioRxiv 10.1101/2023.09.29.559344
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