CCM2_W421AD422A_eGFP
(Plasmid #208880)

• Purpose: Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.
• Depositing Lab: Guillaume Jacquemet
• Publication: Neil J. et al BioRxiv

Backbone

• Vector backbone: pcDNA3.1(+)-N-eGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CCM2
• Species: H. sapiens (human)
• Mutation: mutations W421A and D422A
• Entrez Gene: CCM2 (a.k.a. C7orf22, OSM, PP10187)
• Tag / Fusion Protein:
  • eGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • 10_CCM2_W421AD422A_pcDNA3.1(+)-N-eGFP.gb
• A portion of this plasmid was derived from a plasmid made by: This construct was purchased from GenScript. Briefly, the gene fragments were synthesized using gene synthesis and cloned into pcDNA3.1(+)-N-eGFP using the BamHI/XhoI sites.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry