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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)
(Plasmid #208872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NAP1
  • Species
    H. sapiens (human)
  • Mutation
    S37K/A44E (disrupts NDP52 binding)
  • Entrez Gene
    AZI2 (a.k.a. AZ2, NAP1, TILP)
  • Tag / Fusion Protein
    • MBP-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E) was a gift from Sascha Martens (Addgene plasmid # 208872 ; http://n2t.net/addgene:208872 ; RRID:Addgene_208872)

  • For your References section:

    Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S. bioRxiv September 25, 202 10.1101/2023.09.25.559255
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