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Addgene

pET26-MBP-GLIC
(Plasmid #20887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET26b
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
  • Species
    Gloeobacter violaceus PCC 7421
  • Insert Size (bp)
    2127
  • Tags / Fusion Proteins
    • pelB (N terminal on backbone)
    • His (N terminal on insert)
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26-MBP-GLIC was a gift from Raimund Dutzler (Addgene plasmid # 20887 ; http://n2t.net/addgene:20887 ; RRID:Addgene_20887)

  • For your References section:

    Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel. Hilf RJ, Dutzler R. Nature. 2009 Jan 1. 457(7225):115-8. 10.1038/nature07461 PubMed 18987630
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