pSSA-PMG (pXK-1649)
(Plasmid
#208857)
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Purpose(Empty Backbone) SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSSA-PMG (pXK-1649) was a gift from Kun Xu (Addgene plasmid # 208857 ; http://n2t.net/addgene:208857 ; RRID:Addgene_208857) -
For your References section:
Efficient CRISPR/Cas9-mediated gene editing in mammalian cells by the novel selectable traffic light reporters. Lyu M, Sun Y, Yan N, Chen Q, Wang X, Wei Z, Zhang Z, Xu K. Int J Biol Macromol. 2023 Jul 15;243:124926. doi: 10.1016/j.ijbiomac.2023.124926. Epub 2023 May 20. 10.1016/j.ijbiomac.2023.124926 PubMed 37217056
Map uploaded by the depositor.
