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Addgene

pLenti_CMV:Flag-KaNLuc
(Plasmid #208855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KaNLuc LumiFluor
  • Alt name
    LSSmKate2-NanoLuc BRET fusion
  • Species
    Synthetic
  • Insert Size (bp)
    1227
  • Promoter CMV
  • Tag / Fusion Protein
    • 1xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_CMV:Flag-KaNLuc was a gift from Antonio Amelio (Addgene plasmid # 208855 ; http://n2t.net/addgene:208855 ; RRID:Addgene_208855)

  • For your References section:

    Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters. Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, Kirkland TA, Brennan CK, Prescher JA, Amelio AL. iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. eCollection 2024 Sep 20. 10.1016/j.isci.2024.110655 PubMed 39252965
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