pLenti_CMV:Flag-GpNLuc
(Plasmid
#208840)
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PurposeExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGpNLuc LumiFluor
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Alt nameeGFP-NanoLuc BRET fusion
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SpeciesSynthetic
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Insert Size (bp)1245
- Promoter CMV
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Tag
/ Fusion Protein
- 1xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_CMV:Flag-GpNLuc was a gift from Antonio Amelio (Addgene plasmid # 208840 ; http://n2t.net/addgene:208840 ; RRID:Addgene_208840) -
For your References section:
Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters. Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, Kirkland TA, Brennan CK, Prescher JA, Amelio AL. iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. eCollection 2024 Sep 20. 10.1016/j.isci.2024.110655 PubMed 39252965