pLL3.7-hSt3Cas9 (pXK-1721)
(Plasmid
#208828)
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Purpose(Empty Backbone) CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL3.7
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Vector typeMammalian Expression, CRISPR ; stCas9
- Promoter CMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL3.7-hSt3Cas9 (pXK-1721) was a gift from Kun Xu (Addgene plasmid # 208828 ; http://n2t.net/addgene:208828 ; RRID:Addgene_208828) -
For your References section:
Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus. Xu K, Ren C, Liu Z, Zhang T, Zhang T, Li D, Wang L, Yan Q, Guo L, Shen J, Zhang Z. Cell Mol Life Sci. 2015 Jan;72(2):383-99. doi: 10.1007/s00018-014-1679-z. Epub 2014 Jul 20. 10.1007/s00018-014-1679-z PubMed 25038777
Map uploaded by the depositor.
