pScRad52-SpCas9 (pXK-1646)
(Plasmid
#208823)
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Purpose(Empty Backbone) CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL3.7
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Vector typeMammalian Expression, CRISPR ; Cas9
- Promoter CMV
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Tag
/ Fusion Protein
- Rad52 (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pScRad52-SpCas9 (pXK-1646) was a gift from Kun Xu (Addgene plasmid # 208823 ; http://n2t.net/addgene:208823 ; RRID:Addgene_208823) -
For your References section:
Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Shao S, Ren C, Liu Z, Bai Y, Chen Z, Wei Z, Wang X, Zhang Z, Xu K. Int J Biochem Cell Biol. 2017 Nov;92:43-52. doi: 10.1016/j.biocel.2017.09.012. Epub 2017 Sep 18. 10.1016/j.biocel.2017.09.012 PubMed 28928041
Map uploaded by the depositor.
