pACBE-ASR (pXK-607)
(Plasmid
#208822)
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Purpose(Empty Backbone) Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlue
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Vector typeMammalian Expression
- Promoter CMV, U6
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACBE-ASR (pXK-607) was a gift from Kun Xu (Addgene plasmid # 208822 ; http://n2t.net/addgene:208822 ; RRID:Addgene_208822) -
For your References section:
Development of a universal antibiotic resistance screening reporter for improving efficiency of cytosine and adenine base editing. Ma L, Xing J, Li Q, Zhang Z, Xu K. J Biol Chem. 2022 Jul;298(7):102103. doi: 10.1016/j.jbc.2022.102103. Epub 2022 Jun 6. 10.1016/j.jbc.2022.102103 PubMed 35671823