Skip to main content
Addgene

pG0A0-1-1-PBAD-dCas9
(Plasmid #208815)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Synthetic backbone
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
  • Species
    Synthetic
  • Promoter Arabinsoe-inducible promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTGTCCATATTGCATCAGAC
  • 3′ sequencing primer GTCTTTCGACTGAGCCTTTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG0A0-1-1-PBAD-dCas9 was a gift from Marc Erhardt (Addgene plasmid # 208815 ; http://n2t.net/addgene:208815 ; RRID:Addgene_208815)

  • For your References section:

    A versatile regulatory toolkit of arabinose-inducible artificial transcription factors for Enterobacteriaceae. Naseri G, Raasch H, Charpentier E, Erhardt M. Commun Biol. 2023 Oct 3;6(1):1005. doi: 10.1038/s42003-023-05363-3. 10.1038/s42003-023-05363-3 PubMed 37789111
Related items: