pCFT7.2
(Plasmid
#208808)
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PurposeExpresses the fluorescent protein FAST
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUdO4a
- Backbone size w/o insert (bp) 2892
- Total vector size (bp) 3272
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFAST
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Alt nameFAST1, Y-FAST
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Insert Size (bp)378
- Promoter tac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13-rev
- 3′ sequencing primer M13-fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFT7.2 was a gift from François-Xavier Campbell-Valois (Addgene plasmid # 208808 ; http://n2t.net/addgene:208808 ; RRID:Addgene_208808) -
For your References section:
Anaerobic fluorescent reporters for live imaging of Pseudomonas aeruginosa. Tchagang CF, Mah TF, Campbell-Valois FX. Front Microbiol. 2023 Oct 20;14:1245755. doi: 10.3389/fmicb.2023.1245755. eCollection 2023. 10.3389/fmicb.2023.1245755 PubMed 37928662