pMX1463
(Plasmid
#208805)
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PurposeExpresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5485
- Total vector size (bp) 6666
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB
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Alt namemTOR
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SpeciesH. sapiens (human)
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Insert Size (bp)324
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GenBank IDNM_004958
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Entrez GeneCRP (a.k.a. PTX1)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX1463 was a gift from Linda Wordeman (Addgene plasmid # 208805 ; http://n2t.net/addgene:208805 ; RRID:Addgene_208805) -
For your References section:
Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins. Wordeman L, Decarreau J, Vicente JJ, Wagenbach M. Mol Biol Cell. 2016 Apr 15;27(8):1300-9. doi: 10.1091/mbc.E15-11-0803. Epub 2016 Feb 24. 10.1091/mbc.E15-11-0803 PubMed 26912793