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Addgene

pLVX-opto-hCaspase-8
(Plasmid #208786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caspase 8
  • Species
    H. sapiens (human)
  • Mutation
    CARD domain is removed
  • Entrez Gene
    CASP8 (a.k.a. ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5)
  • Tag / Fusion Protein
    • Cry2 mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-opto-hCaspase-8 was a gift from Petr Broz (Addgene plasmid # 208786 ; http://n2t.net/addgene:208786 ; RRID:Addgene_208786)

  • For your References section:

    Optogenetic activators of apoptosis, necroptosis, and pyroptosis. Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz P. J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 10.1083/jcb.202109038. Epub 2022 Apr 14. 10.1083/jcb.202109038 PubMed 35420640
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