pLVX-opto-hCaspase-4 dCARD
(Plasmid #208785)

• Purpose: Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2
• Depositing Lab: Petr Broz
• Publication: Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 10.1083/jcb.202109038. Epub 2022 Apr 14.

Backbone

• Vector backbone: pLVX
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cry2 mCherry Caspase4 dCARD
• Species: H. sapiens (human)
• Mutation: CARD domain is removed
• Entrez Gene: CASP4 (a.k.a. ICE(rel)II, ICEREL-II, ICH-2, Mih1, Mih1/TX, TX)
• Tag / Fusion Protein:
  • Cry2 mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TTGGTCACCTTCAGCTTGG

Resource Information

• Supplemental Documents:
  • P665.annotation.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry