pLVX-opto-mCasp8
(Plasmid
#208782)
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PurposeInducibly expresses mouse caspase8 fused to light-activatable Cry2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry mCaspase8 Cry2
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SpeciesM. musculus (mouse)
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Entrez GeneCasp8 (a.k.a. CASP-8, FLICE, MACH, Mch5)
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Tags
/ Fusion Proteins
- mCherry (N terminal on insert)
- Cry2 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TTGGTCACCTTCAGCTTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-opto-mCasp8 was a gift from Petr Broz (Addgene plasmid # 208782 ; http://n2t.net/addgene:208782 ; RRID:Addgene_208782) -
For your References section:
Optogenetic activators of apoptosis, necroptosis, and pyroptosis. Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz P. J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 10.1083/jcb.202109038. Epub 2022 Apr 14. 10.1083/jcb.202109038 PubMed 35420640
