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Addgene

MSCV-mNINJ1-D53A-GFP
(Plasmid #208778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NINJ1
  • Species
    M. musculus (mouse)
  • Mutation
    Changed aspartic acid 53 to alanine (D53A)
  • Entrez Gene
    Ninj1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-mNINJ1-D53A-GFP was a gift from Petr Broz (Addgene plasmid # 208778 ; http://n2t.net/addgene:208778 ; RRID:Addgene_208778)

  • For your References section:

    NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis. Ramos S, Hartenian E, Santos JC, Walch P, Broz P. EMBO J. 2024 Apr;43(7):1164-1186. doi: 10.1038/s44318-024-00055-y. Epub 2024 Feb 23. 10.1038/s44318-024-00055-y PubMed 38396301
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