pLVX-mNINJ1-GFP
(Plasmid
#208775)
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PurposeInducibly expresses mouse NINJ1 tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNINJ1
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SpeciesM. musculus (mouse)
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Entrez GeneNinj1
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TTGGTCACCTTCAGCTTGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-mNINJ1-GFP was a gift from Petr Broz (Addgene plasmid # 208775 ; http://n2t.net/addgene:208775 ; RRID:Addgene_208775) -
For your References section:
NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis. Ramos S, Hartenian E, Santos JC, Walch P, Broz P. EMBO J. 2024 Apr;43(7):1164-1186. doi: 10.1038/s44318-024-00055-y. Epub 2024 Feb 23. 10.1038/s44318-024-00055-y PubMed 38396301