Skip to main content
Addgene

phROSA26-Tet-HA-JSAP1_WT
(Plasmid #208772)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMK365
  • Backbone manufacturer
    Addgene #121185
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JSAP1
  • Alt name
    JIP3
  • Alt name
    MAP8IP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4023
  • Entrez Gene
    MAPK8IP3 (a.k.a. JIP-3, JIP3, JSAP1, NEDBA, SYD2, syd)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTTTGCTTATGTAAACCAGG
  • 3′ sequencing primer attaagggttattgaatatgatcgCCCCGAAAAGTGCCACCTGACGTCG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phROSA26-Tet-HA-JSAP1_WT was a gift from Katsuji Yoshioka (Addgene plasmid # 208772 ; http://n2t.net/addgene:208772 ; RRID:Addgene_208772)

  • For your References section:

    Overexpression of JNK-associated leucine zipper protein induces chromosomal instability through interaction with dynein light intermediate chain 1. Suzuki R, Kanemaki MT, Suzuki T, Yoshioka K. Genes Cells. 2023 Nov 14. doi: 10.1111/gtc.13083. 10.1111/gtc.13083 PubMed 37963657
Related items: