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pRSFDuet1_CylLS_CylM
(Plasmid #208760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet1
  • Total vector size (bp) 6922
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CylLS
  • Alt name
    Cytolysin S
  • Species
    Synthetic
  • Insert Size (bp)
    237
  • Promoter T7
  • Tag / Fusion Protein
    • Hisx6 Tag (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer cttatgcgactcctgcattaggaa
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CylLM
  • Alt name
    Lanthionine synthetase CylLM
  • Species
    Synthetic
  • Insert Size (bp)
    2970
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer cgtattgtacacggccgcataatc
  • 3′ sequencing primer gctagttattgctcagcgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet1_CylLS_CylM was a gift from Wilfred van der Donk (Addgene plasmid # 208760 ; http://n2t.net/addgene:208760 ; RRID:Addgene_208760)

  • For your References section:

    Facile Method for Determining Lanthipeptide Stereochemistry. Luo Y, Xu S, Frerk AM, van der Donk WA. Anal Chem. 2024 Jan 30;96(4):1767-1773. doi: 10.1021/acs.analchem.3c04958. Epub 2024 Jan 17. 10.1021/acs.analchem.3c04958 PubMed 38232355
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