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Addgene

pSR1054
(Plasmid #208740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCM29
  • Backbone size w/o insert (bp) 6603
  • Total vector size (bp) 6567
  • Modifications to backbone
    Changed promoter expressing sfgfp to PAmy
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmy
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    45
  • Promoter PAmy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer aaatcgacactgaatttgc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chloramphenicol resistance in S. aureus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR1054 was a gift from Lauren Andrews (Addgene plasmid # 208740 ; http://n2t.net/addgene:208740 ; RRID:Addgene_208740)

  • For your References section:

    Toolbox of Characterized Genetic Parts for Staphylococcus aureus. Rondthaler SN, Sarker B, Howitz N, Shah I, Andrews LB. ACS Synth Biol. 2023 Dec 8. doi: 10.1021/acssynbio.3c00325. 10.1021/acssynbio.3c00325 PubMed 38064657
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