pMSCV-FlagMLL-pl-ENL(5613)
(Plasmid #20873)

• Depositing Lab: Robert Slany
• Publication: Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Backbone

• Vector backbone: pMSCV-neo
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MLL
• Alt name: mixed-lineage leukemia
• Alt name: ENL, mixed lineage leukemia eleven nineteen leukemia
• Species: H. sapiens (human)
• Insert Size (bp): 4580
• Entrez Gene: KMT2A (a.k.a. ALL-1, ALL1, CXXC7, GAS7, HRX, HTRX, HTRX1, MLL, MLL1, MLL1A, TRX1, WDSTS)
• Entrez Gene: MLLT1 (a.k.a. ENL, LTG19, YEATS1)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: MSCV
• 3′ sequencing primer: MSCV-rev

Resource Information

• Supplemental Documents:
  • pMSCV-FlagMLL-pl-ENL(5613) sequence
• A portion of this plasmid was derived from a plasmid made by: Francis Stewart, University Durden Germany
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.

SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.