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Addgene

pAAV-EF1a-DIO-GRAB_g5-HT2m
(Plasmid #208722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5383
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green fluorescent 5-HT sensor GRAB_g5-HT2m
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2151
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-GRAB_g5-HT2m was a gift from Yulong Li (Addgene plasmid # 208722 ; http://n2t.net/addgene:208722 ; RRID:Addgene_208722)

  • For your References section:

    Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. Deng F, Wan J, Li G, Dong H, Xia X, Wang Y, Li X, Zhuang C, Zheng Y, Liu L, Yan Y, Feng J, Zhao Y, Xie H, Li Y. bioRxiv 2023.05.27.542566 10.1101/2023.05.27.542566
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