pAAV-hSyn-DIO-GRAB_g5-HT2h
(Plasmid #208715)

• Purpose: Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase
• Depositing Lab: Yulong Li
• Publication: Deng et al bioRxiv 2023.05.27.542566

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 4495
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Green fluorescent 5-HT sensor GRAB_g5-HT2h
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 2151
• Promoter: hSyn

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.05.27.542566v1 for bioRxiv preprint.