pDisplay-GRAB_r5-HT1.0
(Plasmid
#208713)
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PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208713 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDisplay
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Backbone manufacturerpDisplay
- Backbone size w/o insert (bp) 6550
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRed fluorescent 5-HT sensor GRAB_r5-HT1.0
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)1992
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.05.27.542566v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-GRAB_r5-HT1.0 was a gift from Yulong Li (Addgene plasmid # 208713 ; http://n2t.net/addgene:208713 ; RRID:Addgene_208713) -
For your References section:
Dual-color GRAB sensors for monitoring spatiotemporal serotonin release in vivo. Deng F, Wan J, Li G, Dong H, Xia X, Wang Y, Li X, Zhuang C, Zheng Y, Liu L, Yan Y, Feng J, Zhao Y, Xie H, Li Y. bioRxiv 2023.05.27.542566 10.1101/2023.05.27.542566