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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK+
- Backbone size w/o insert (bp) 2935
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAny commonly used strains
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKanamycinResistance-RpsL
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Alt nameKanaRpsL
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Alt namestreptomycin
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SpeciesE.coli
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Insert Size (bp)1743
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Mutationnone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSK+KanaRpsL was a gift from Jiyue Zhu (Addgene plasmid # 20871 ; http://n2t.net/addgene:20871 ; RRID:Addgene_20871) -
For your References section:
A New Positive/Negative Selection Scheme for Precise BAC Recombineering. Wang S, Zhao Y, Leiby M, Zhu J. Mol Biotechnol. 2009 Jan 22. ():. 10.1007/s12033-009-9142-3 PubMed 19160076