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Addgene

pSK+KanaRpsL
(Plasmid #20871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone size w/o insert (bp) 2935
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Any commonly used strains
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KanamycinResistance-RpsL
  • Alt name
    KanaRpsL
  • Alt name
    streptomycin
  • Species
    E.coli
  • Insert Size (bp)
    1743
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSK+KanaRpsL was a gift from Jiyue Zhu (Addgene plasmid # 20871 ; http://n2t.net/addgene:20871 ; RRID:Addgene_20871)

  • For your References section:

    A New Positive/Negative Selection Scheme for Precise BAC Recombineering. Wang S, Zhao Y, Leiby M, Zhu J. Mol Biotechnol. 2009 Jan 22. ():. 10.1007/s12033-009-9142-3 PubMed 19160076
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