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Addgene

pminiTol2-HuC-NE2m
(Plasmid #208688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2
  • Backbone size w/o insert (bp) 13381
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
  • Alt name
    GRAB_NE2m
  • Alt name
    NE2m
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1998
  • Promoter Huc

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAAGCATTACACCCCTC
  • 3′ sequencing primer TGTTTCAGGTTCAGGGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pminiTol2-HuC-NE2m was a gift from Yulong Li (Addgene plasmid # 208688 ; http://n2t.net/addgene:208688 ; RRID:Addgene_208688)
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